https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:55745 Wed 19 Jun 2024 15:38:25 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:55746 300% after 1 week) under mixotrophic conditions. While transitioning to the second week, there was a change in growth pattern, with >600% increase in chlorophyll content and minor pH alterations, indicating the phenotypic response to the synergistic effect of nutritional mode during wastewater treatment. Both the strains showed a similar removal (70–80%) of both organic and inorganic carbon present in the wastewater, while the removal of nitrogen and phosphate (80–90%) was significant during mixotrophy. Metabolic analysis revealed the prominence of key pathways such as CO2 fixation, nitrogen assimilation, and amino acid metabolism under mixotrophic conditions of an open system. Especially, strain MAS1 exhibited elevated expression of organic acids, suggesting their crucial role in nutrient uptake from winery effluents during mixotrophy. Thus, our study demonstrates that a synergy of wastewater environment and nutritional mode enhances phenotypic trait expression in microalgae to improve metabolic flexibility and sustainable biomass production.]]> Wed 19 Jun 2024 15:37:26 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44477 Desmodesmus sp. MAS1 and Heterochlorella sp. MAS3, were assessed for their ability in iron (Fe) removal from an AMD sample in nonimmobilized and immobilized systems. Use of free and immobilized cells exhibited 46−48% and 65−79% Fe removal, respectively, after 48 h of incubation. Compared with free cells, immobilized cells exhibited no apparent changes in morphology and granularity, as revealed by flow cytometry analysis, after their exposure to AMD samples. The second derivative spectra from Fourier transform infrared spectroscopy showed vibrational stretching for proteins and hydroxyl groups in immobilized cells. Thus, the immobilization technology offers a protective mechanism in acid-adapted strains against Fe present in AMD samples. Analysis of the immobilized acid-adapted microalgal technology by life cycle assessment (LCA) revealed its environmental sustainability because of less contribution to global warming and limited fossil fuel consumption. We demonstrated that the immobilized acid-adapted microalgal technology is much superior to calcined eggshell−microalgal or conventional limestone systems indicated in the literature for AMD treatment. Thus, this is the first study describing the potential application of microalgal cells entrapped in alginate beads in a greener and economical approach to treat AMD for sustainable mining.]]> Wed 07 Feb 2024 16:37:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48292 90%) to the overall emergy and were much lower than in passive and active treatment systems. The microalgal treatment required 2–15 times more renewable inputs than the other two treatment systems. Additionally, the emergy indices indicated higher environmental loading ratio and lower per cent renewability, suggesting the need for adequate renewable inputs in the immobilized microalgal system. The emergy yield ratio for biodiesel production from the microalgal biomass after AMD treatment was >1.0, which indicates a better emergy return on total emergy spent. Based on greenhouse gas emissions, carbon footprint analysis (CFA), was performed using default emission factors, in accordance with the IPCC standards and the National Greenhouse Energy Reporting (NGER) program of Australia. Interestingly, CFA of acid-adapted microalgal technology revealed significant greenhouse gas emissions due to usage of various construction materials as per IPCC, while SCOPE 2 emissions from purchased electricity were evident as per NGER. Our findings indicate that the immobilized microalgal technology is highly sustainable in AMD treatment, and its potential could be realized further by including solar energy into the overall treatment system.]]> Wed 07 Feb 2024 14:55:19 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54438 Tue 27 Feb 2024 13:56:17 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:55796 Sat 22 Jun 2024 12:47:32 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54930 Fri 22 Mar 2024 14:32:28 AEDT ]]>